Faculty Profile

Enoch P. Baldwin
Associate Professor
Molecular and Cellular Biology (College of Biological Sciences)
Chemistry Department
4B Briggs
Office 752-1108
Lab 752-5235
epbaldwin@ucdavis.edu
http://www-crystal.ucdavis.edu/talent/baldwinlab/PAPERS
[Picture of Enoch P. Baldwin]
Structural Biochemistry, Enzymology and Protein Engineering of Protein-nucleic acid Interactions. My lab uses X-ray crystallography, biochemistry and molecular genetics to dissect and engineer mechanisms of enzymes involved in nucleic acid metabolism. A primary focus is understanding the basis for the DNA specificity of Cre site-specific recombinase, and using this understanding to obtain variants that recognize alternative DNA sequences. A second focus is on the mechanism and regulation of Cytidine Triphosphate Synthetase, the ultimate enzyme for pyrimidine biosynthesis, and a target for anti-cancer and anti-parasitic drugs.

Degrees:
1989 - PhD - University of California, Berkeley - Chemistry
1982 - BA - State University of New York, Purchase - Chemistry

Awards:
2006, "Teacher of the Year", Biochemistry and Molecular Biology Graduate Group

Department and Center Affiliations:
Department of Chemistry
Molecular and Cellular Biology

Professional Societies:
American Chemical Society
Protein Society
AAAS

Grad Group Affiliations and Specialties:
Biochemistry and Molecular Biology
Biophysics
Microbiology
Non-DBS Grad Group(s) - Chemistry

Publications:
Gelato, K.A, Martin, S.S, Liu, P.H., Saunders, A.A., and E.P. Baldwin. 2008. Spatially-Directed Assembly of a Heterotetrameric Cre-Lox Synpase Restricts Recombination Specificity. J. Mol Biol. 385, 653-665.

Chang, Y.F., Martin, S.S., Baldwin, E.P., & Carman, G.M. 2007. Phosphorylation of Human CTP Synthetase 1 by Protein Kinase C: Identification of Ser462 and Thr455 as major sites of phosphorylation. J. Biol. Chem. 282, 17613-22.

Gelato, K.A., Martin, S.S., Wong, S., and Baldwin, E.P. 2006. Mulitple Levels of Affinity-Dependent DNA Discrimination in Cre-LoxP Recombination. Biochemistry 45, 12216-26.

Gelato, K.A., Martin, S.S., Baldwin, E.P. 2005. Reversed DNA Strand Cleavage Specificity in Initiation of Cre-LoxP Recombination Induced by the His289Ala Active Site Substitution. J. Mol. Biol. 354, 233-45.

Endrizzi, J.A., Kim, H., Anderson, P.M., Baldwin, E.P. 2005. Mechanisms of product feedback regulation and drug resistance in cytidine triphosphate synthetases from the structure of a CTP-inhibited complex. Biochemistry 44, 13491-9.

Han, G.-S. , Sreenivas, A., Choi, M.-G. , Chang, Y.-F., Martin, S.S. , Baldwin, E.P., and G. M. Carman. 2005. Expression of Human CTP Synthetase in Saccharomyces cerevisiae Reveals Phosphorylation by Protein Kinase A, J. Biol. Chem. 280, 38328-36.

Endrizzi, J.A., Kim, H., Anderson, P.M., Baldwin, E.P. 2004. Crystal Structure of Escherichia coli Cytidine Triphosphate Synthetase, a Nucleotide-Regulated Glutamine Amidotransferase/ATP-Dependent Amidoligase Fusion Protein and Homologue of Anticancer and Antiparasitic Drug Targets. Biochemistry 43, 6447-63.

Friddle, RW, Klare, JE, Martin, SS, Corzett, M, Balhorn, R, Baldwin, EP, Baskin, RJ, and A Noy. 2004. Mechanism of DNA compaction by yeast mitochondria protein ABF2p. Biophysical J. 86, 1632-9.

Baldwin EP, Martin SS, Abel J, Gelato KA, Kim H, Schultz PG, and SS Santoro. 2003. A specificity switch in selected Cre recombinase variants is mediated by macromolecular plasticity and water. Chem. Biol. 110, 1085-93.

Martin SS, Wachi S, and EP Baldwin. 2003. Vanadate-based transition-state analog inhibitors of Cre-LoxP recombination. Biochem. Biophys Res. Comm. 308: 529-34.

Brewer, LR, Friddle, R, Noy, A, Baldwin, E, Martin, SS, Corzett, M, Balhorn, R and RJ Baskin. 2003. Packaging of Single DNA Molecules by the Yeast Mitochondrial Protein ABF2p. Biophysical J. 85, 2519-24.

Martin SS, Chu VC, and EP Baldwin. 2003. Modulation of active complex assembly and turnover rate by protein-DN Interactions in Cre-LoxP recombination. Biochemistry 42: 6814-6826.

Martin SS, Pulido E, Chu, VC, Lechner TS, and EP Baldwin. 2002. The order of strand exchanges in Cre-LoxP recombination and its basis suggested by the crystal structure of a Cre-LoxP Holliday junction complex. J. Mol. Biol. 319: 107-127.

Denison, MS, Pandini, A, Nagy, SR, Baldwin, EP, and Bonati, L. 2002. Ligand binding and activation of the Ah receptor. Chem. Biol. Interact. 141: 3-24.

Woods KC, Martin SS, Chu VC and EP Baldwin. 2001. Quasi-equivalence in site-specific recombinase structure and function:crystal structure and activity of trimeric Cre recombinase bound to a three-way Lox DNA junction. J. Mol. Bio. 313:49-69

Research Interests:
X-ray crystallography, protein and DNA structure and function, and protein engineering, focussing on enzymology and molecular recognition. Model systems include DNA and nucleotide binding protieins and enzymes.

Laboratory Personnel:
Baldwin Lab - Shelley Martin (1997-2007), Kathy Gelato (2001-2007), Hanseong Kim (2001-2005), Dr. James Endrizzi (2002-2006), Lesley Steinman (2004-2007), Roger Jessinghaus (2008-)
http://www-crystal.ucdavis.edu/talent/baldwinlab/index.html

Teaching Interests:


Macromolecular structure and function, macromolecular interactions, protein sequence-structure-function relationships, enzyme mechanisms, X-ray structure determination.

Courses Taught:
BMB 221A Physical Biochemistry (5 Lectures) - Term(s): Fall
BMB 221C Molecular Biology (3 Lectures) - Term(s): Spring
MCB 138 Current topics in DNA/protein interactions - Term(s): Spring
BMB 221B Mechanistic Enzymology (every other year) - Term(s): Fall
MCB 124 Macromolecular Structure and Function - Term(s): Fall