Faculty Profile
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Dan Kliebenstein
Assistant Professor Plant Sciences 129 Asmundson Hall Office 530-754-7775 Lab http://www.plantsciences.ucdavis.edu/kliebenstein/ |
Degrees:
1999 - PhD - Cornell University - Genetics
1993 - BS - Iowa State University - Genetics
Department and Center Affiliations:
Department of Plant Sciences
Grad Group Affiliations and Specialties:
Biochemistry and Molecular Biology
Genetics
Plant Biology
Publications:
Zhang, Z., Ober, J.A. and D.J. Kliebenstein. (2006) The Gene Controlling the Quantitative Trait Locus EPITHIOSPECIFIER MODIFIER1 Alters Glucosinolate Hydrolysis and Insect Resistance in Arabidopsis. Plant Cell 18(6):1524-36.
Kliebenstein, D.J., West, M.A.L, van Leeuwen, H., Kim, K., Doerge, R.W., and D.A. St. Clair. (2006) Identification of QTL Controlling Gene Expression Networks Defined A Prioiri. BMC Bioinformatics 7(1):308.
Kliebenstein, D.J., West, M.A.L., van Leeuwen, H., Kim, K., Doerge, R.W., Michelmore, R.W. and D.A. St.Clair. (2006) Genomic survey of gene expression diversity in Arabidopsis thaliana. Genetics 172(2):1179-7789.
West, M.A.L, van Leeuwen, H., Kozik, A., Kliebenstein, D.J., Doerge, R.W., St. Clair, D.A. and R.W. Michelmore. (2006) High-density haplotyping with microarray-based expression and single feature polymorphism markers in Arabidopsis. Genome Research 16(6):787-95.
Brown, B.A., Cloix, C., Jiang, G.H., Kaiserli, E., Herzyk, P., Kliebenstein, D.J. and G.I. Jenkins. (2005) A UV-B-specific signaling component orchestrates plant UV-protection. (In press) Proc. Natl. Acad. Sci. USA
Kliebenstein, D.J., Rowe, H.C. and K.J. Denby. (2005) Secondary metabolites influence Arabidopsis/Botrytis interactions: variation in host production and pathogen susceptibility. Plant Journal 44(1):25-36.
Windsor, A.J., Reichelt, M., Figuth, A., Svatoa, A., Kroymann, J., Kliebenstein, D.J., Gershenzon, J. and T. Mitchell-Olds. (2005) Geographic and evolutionary diversification of glucosinolates among near relatives of Arabidopsis thaliana (Brassicaceae). Phytochemistry 66(11):1321-1333.
Kliebenstein, D.J., Kroymann, J., and T. Mitchell-Olds. (2005) The glucosinolate-myrosinase system in an ecological and evolutionary context. Current Opinion in Plant Biology 8(3):264-271.
Murray, S.L., Adams, N., Kliebenstein, D.J., Loake, G.J. and K.J. Denby. (2005) A constitutive PR-1::Luciferase expression screen identifies Arabidopsis mutants with differential disease resistance to biotrophic and necrotrophic pathogens. Molecular Plant Pathology 6(1):31-41.
Kliebenstein, D.J. (2004) Secondary metabolites and plant/environment interactions: a view through Arabidopsis thaliana tinged glasses. Plant Cell and Environment 27(6):675-684.
Denby, KJ, Kumar, P. and Kliebenstein, D.J. (2004) Identification of Botrytis cinerea susceptibility loci in Arabidopsis thaliana. Plant Journal 38(3):473-486.
Wittstock, U., Kliebenstein, D.J., Lambrix, V., Reichelt, M. and J. Gershenzon. (2003) Glucosinolate hydrolysis and its impact on generalist and specialist insect herbivores. Rec Adv Phytochem 37: 101-125 (eds. Romeo JT, Dixon RA, Vol. 37: Integrative Phytochemistry: From Ethnobotany to Molecular Ecology. Pergamon, Amsterdam)
Lambrix VM, Reichelt M, Mitchell-Olds T, Kliebenstein DJ and J Gershenzon. 2001. The Arabidopsis epithiospecifier protein promotes the hydrolysis of glucosinolates to nitriles and influences Trichoplusia in herbivory. Plant Cell. 13:2793-280
Kliebenstein DJ, Pedersen D and T Mitchell-Olds. 2002. Comparative analysis of insect resistance QTL and QTL controlling the myrosinase/glucosinolate system in Arabidopsis thaliana. Genetics. 161:325-332
Kliebenstein DJ, Lim JE, Landry LG and RL Last. 2002. Arabidopsis UVR8 regulates Ultraviolet-B signal transduction and tolerance and contains sequence similarity to Human Regulator of Chromatin Condensation 1. Plant Physiology. 130: 234-243
Research Interests:
My laboratory studies how small metabolites called "secondary metabolites" control two plant phenotypes. 1) The nutritional value of the plant in a human diet. 2) The resistance of plants to environmental stress.
As plants cannot physically move to evade environmental attacks such as UV irradiation or insect herbivory, they have evolved a complex set of biochemicals to provide protection. These chemicals are broadly classified as "secondary metabolites" and in addition to protecting plants are often important components for human nutrition. The same secondary metabolites can prevent insect herbivory on a plant and when in human diet can help to prevent cancer formation.
Most phenotypes are controlled through the interaction of a large set of genes. The difference in phenotype between two individuals is often controlled by polymorphisms at a number of these genes simultaneously. To understand how what these genes are and how they interact it is necessary to use quantitative genetic techniques. Further, quantitative genetics is the basis for a wide range of fields including human disease genetics and plant/animal breeding.
Modern genomics technologies such as transcriptomics and proteomics allow researchers to rapidly study the level and identity of 10's of thousands of mRNAs and proteins in a given sample. However the important question is how the mRNA and proteins combine to generate a phenotype. The link between proteins and phenotype is small metabolites. Recently, techniques such as metabolomics and high-throughput metabolite profiling have become available that allow rapid measuring of thousands of small metabolites. My lab is utilizing these technologies to understand how changes in small metaoblites influence plant/environment interactions.
Natural variation is simply the difference in phenotype between two individuals of a given species. This difference is due to molecular polymorphisms in specific genes that control the given phenotype. However, despite this simple origin, these polymorphisms are extremely important. In humans, their presence or absence will determine the tendency of an individual to develop cancer or whether a specific drug will work for that individual. In agricultural system, these polymorphisms control whether or not a crop will be succesfull in a given year. We studying how natural variation in plants controls their nutritional quality for humans and how it controls the plants response to environmental stress.
Teaching Interests:
Natural variation and secondary metabolites.
Courses Taught:
BIS 101 Genes and Gene Regulation - Term(s): Winter