Faculty Profile

Martha E. O'Donnell
Professor of Physiology and Membrane
Physiology and Membrane Biology
4155 Tupper Hall
Office 752-7626
Lab
meodonnell@ucdavis.edu
http://dev.physiology.ucdavis.edu/odonnell/index.html
[Picture of Martha E. O'Donnell]
Stroke, cerebral edema, estrogen, brain microvascular endothelial cells, astrocytes, cotransport

Degrees:
- - - B.S. University of California, Davis
- - - Ph.D. University of California, Davis
- - - Postdoctoral fellowship University of Chicago

Department and Center Affiliations:
Department of Physiology and Membrane Biology

Professional Societies:
American Physiological Society
American Society for Cell Biology
Microcirculatory Society
North American Vascular Biology Organization
American Heart Association Stroke Council
Society for Neuroscience
International Society for Cerebral Blood Flow and Metabolism

Grad Group Affiliations and Specialties:
Cell and Developmental Biology
Molecular, Cellular, and Integrative Physiology

Publications:
O'Donnell, M.E., A. Martinez and D. Sun (1995) Cerebral microvascular endothelial cell Na-K-Cl cotransport: regulation by astrocyte-conditioned medium. American Journal of Physiology 268: C747-C754.

O'Donnell, M.E., J.D. Brandt, and F.E. Curry (1995) Na-K-Cl cotransport regulates intracellular volume and monolayer permeability of trabecular meshwork cells. American Journal of Physiology, 268: C1067-C1074.

Sun, D., C. Lytle and M.E. O'Donnell (1995) Astroglial cell-induced expression of Na-K-Cl cotransporter in brain microvascular endothelial cells. American Journal of Physiology, 269: C1506-C1512.

O'Donnell, M.E., A. Martinez and D. Sun (1995) Endothelial Na-K-Cl cotransport regulation by tonicity and hormones: phosphorylation of cotransport protein. American Journal of Physiology, 269: C1513-C1523.

Sun, D. and M.E. O'Donnell (1996) Astroglial-mediated phosphorylation of the Na-K-Cl cotransporter in brain microvessel endothelial cells. American Journal of Physiology, 271: C620-C627.

Sun, D., C. Lytle and M.E. O'Donnell (1997) IL-6 secreted by astroglial cells regulates Na-K-Cl cotransport in brain microvessel endothelial cells. American Journal of Physiology, 272: C1829-C1835.

Putney, L.K., J.D. Brandt, and M.E. O'Donnell (1997) Effects of dexamethasone on sodium-potassium-chloride cotransport in trabecular meshwork cells. Investigative Ophthalmology and Visual Science 38: 1229-1240.

Yerby, T.R., C.R.T. Vibat, D. Sun, J.A. Payne and M.E. O'Donnell (1997) Molecular characterization of the Na-K-Cl cotransporter of bovine aortic endothelial cells. American Journal of Physiology 273: C188-C197.

Kajimura, M., M.E. O'Donnell and F.E. Curry (1997) Effect of cell shrinkage on permeability of cultured bovine aortic endothelia and frog mesenteric capillaries. Journal of Physiology 503: 413-425.

Cai, S., L. Pestic, M.E. O'Donnell, N. Wang, D. Ingber, E. Elson and P. de Lanerolle (1998) Regulation of cytoskeletal mechanics and cell growth by myosin light chain kinase. American Journal of Physiology 275: C1349-C1356.

Putney, L.K., J.D. Brandt and M.E. O'Donnell (1998) Na-K-Cl cotransport in normal and glaucomatous human trabecular meshwork cells. Investigative Ophthalmology and Visual Science 40: 425-434.

Putney, L.K., C.R.T. Vibat and M.E. O'Donnell (1999) Intracellular Cl regulates Na-K-Cl cotransport activity in human trabecular meshwork cells, American Journal of Physiology 277: C373-C383

Al-Aswad, L. A., J. Gong, D. Lee, M.E. O'Donnell, J.D. Brandt, W. J. Ryan and K.A. Erickson (1999) Effects of Na-K-2Cl cotransport regulators on outflow facility in calf and human eyes in vitro. Investigative Ophthalmology and Visual Science 40 (8): 1695-1701.

Brandt, J.D. and M.E. O'Donnell (1999) How does the trabecular meshwork regulate outflow? Clues from the microvascular endothelium, Journal of Glaucoma 8 (5):328-339.

Suvatne, J., A. Barakat and O'Donnell, M.E (2001) Flow-induced expression of endothelial Na-K-Cl cotransport: dependence on K+ and Cl- channels, American Journal of Physiology-Cell Physiology 280: c216-227.

Stamer, W. D., K.C. Peppel, M.E. O'Donnell, B.C. Roberts, F. Wu and D.L. Epstein (2001) Expression of aquaporin-1 in human trabecular meshwork cells: role in resting cell volume, Investigative Ophthalmology and Visual Science 42: 1803-1811.

Vibat, C.R.T, M.J. Holland, J.J. Kang, L.K. Putney and M.E. O'Donnell (2001) Quantitation of Na+-K+-2Cl- cotransport splice variants in human tissues using kinetic PCR, Analytical Biochemistry 298: 218-230.

O'Donnell, M.E., J. Suvatne, P.A. Pappone and J.D. Brandt (2001) Activation of ion conductances in human trabecular meshwork cells by swelling and by niflumic acid. Investigative Ophthalmology and Visual Science 42: S138.

O'Donnell, M.E., L. Tran, T. Lam, X.B. Liu and S.E. Anderson (2004). Bumetanide inhibition of the blood-brain barrier Na-K-Cl cotransporter reduces edema formation in the rat middle cerebral artery occlusion model of stroke. Journal of Cerebral Blood Flow and Metabolism, 24: 1046-1056.

T. I. Lam, S.E. Anderson, N. Glaser and M. E. O'Donnell (2005). Bumetanide reduces cerebral edema formation in rats with diabetic ketoacidosis. Diabetes 54: 510-516.

O'Donnell, M.E., V. Duong, S. Suvatne, S. Foroutan, and D. M. Johnson (2005). Arginine vasopressin stimulation of cerebral microvascular endothelial cell Na-K-Cl cotransport activity is V1 receptor- and [Ca]-dependent. American Journal of Physiology, 289: C283-C292.

Foroutan, S., J. Brillault, B. Forbush, and M.E. O'Donnell (2005). Moderate to severe ischemic conditions increase activity and phosphorylation of the cerebral microvascular endothelial cell Na-K-Cl cotransporter. American Journal of Physiology: Cell Physiology 289: C1492-C1501.

M.E. O’Donnell, T.I. Lam, L. Tran and S.E. Anderson (2005). The role of the blood-brain barrier Na-K-Cl cotransport in stroke. In: P.Lauf and N. Adragna, eds, Cell Volume and Signaling, Advances in Experimental Biology and Medicine, Proceedings of the 2003 International Symposium on Cell Volume Regulation. Vol. 559, pp 67-75, Springer Publishers.

O'Donnell, M.E., T. I. Lam, L.Q. Tran, S. Foroutan and S.E. Anderson (2006). Estradiol reduces activity of the blood-brain barrier Na-K-Cl cotransporter and decreases edema formation in permanent middle cerebral artery occlusion. Journal of Cerebral Blood Flow and Metabolism 26: 1234-1249.

Pedersen, S.F., M.E. O’Donnell, S.E. Anderson, and P.M. Cala (2006). Physiology and pathophysiology of Na+/H+ exchange and Na+/K+/2Cl- cotransporter in the heart, brain, and blood. American Journal of Physiology: Regulatory, Integrative and Comparative Physiology 291; R1-R25.

Brillault, J., T.I. Lam, J.M. Rutkowsky, S. Foroutan and M.E. O’Donnell (2007). Hypoxia effects on cell volume and ion uptake of cerebral microvascular endothelial cells. American Journal of Physiology: Cell Physiology doi: 10.1152/ajpcell.00148.2007.

Chang, E., M.E. O’Donnell and A.I. Barakat. Shear stress and 17?-estradiol modulate cerebral microvascular endothelial Na-K-Cl cotransporter and Na/H exchanger protein levels (2007). American Journal of Physiology: Cell Physiology doi: 10.1152/ajpcell.00045.2007.

Research Interests:
Signal transduction and ion transport processes of the neurovascular unit with an emphasis on brain microvascular endothelial cells. Our studies have focused largely on: 1) the role of Na transporters in normal blood-brain barrier function as well as in the pathophysiology of stroke; and 2) the effects of estrogen on blood-brain barrier and astrocyte Na transporters . A variety of experimental methods are employed, including molecular biology, subcellular fractionation, protein biochemistry, cell culture, microvessel isolation, immunohistochemistry and electron microscopy and nuclear magnetic resonance of whole animals.

Laboratory Personnel:
O'Donnell laboratory, 4152/4149 Tupper Hall - Natalie Yuen, B.S., Tina Lam, Ph.D. student, Jennifer Rakowsky, Ph.D. student, Breanna Wallace, Ph.D. student, Karen Jelks, Postdoctoral Fellow, Jay Chen, Postdoctoral Fellow
http://dev.physiology.ucdavis.edu/

Teaching Interests:


Cellular physiology, Cell signalling, Ion transport, Cerebrovascular physiology, Epithelial cell physiology, G.I. physiology

Courses Taught:
HPH 400 Human Physiology (Co-Instructor of Record) - Term(s): Fall
MCP 210A Advanced Physiology (Physiology Core Course) - Term(s): Fall